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Intermediate

Part:BBa_K558002:Design

Designed by: Jovanna Casas   Group: iGEM11_Nevada   (2011-08-25)


PDC/ADH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3034
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 3035
    Illegal PstI site found at 3049
    Illegal NotI site found at 7
    Illegal NotI site found at 1748
    Illegal NotI site found at 3042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal XhoI site found at 1756
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3035
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3035
    Illegal PstI site found at 3049
    Illegal AgeI site found at 1052
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1577
    Illegal AgeI site found at 2348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 86
    Illegal BsaI site found at 2699
    Illegal SapI site found at 500
    Illegal SapI site found at 1163


Design Notes

none


Source

The sequence for these genes is based on Zymomonas mobilis, a microorganism that naturally produces ethanol.

References